BsrF

• Description: small RNA
  
  

Gene name	bsrF
Synonyms	SR2
Essential	no
Product	small RNA
Function	unknown
Gene length	bp
Immediate neighbours	yobO, csaA
Gene sequence (+200bp)
Genetic context File:GenE context.gif

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: no entry

• SubtiList entry: no entry

• Structure:

Expression and regulation

• Operon: bsrF PubMed

• Sigma factor: SigA PubMed

• Regulation: weakly activated by CodY PubMed

• Regulatory mechanism: CodY: transcription activation PubMed

• Additional information:

Biological materials

• Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab

• Expression vectors:
  • pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene), available in the Sabine Brantl lab
  • pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene), available in the Sabine Brantl lab

• lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab

Labs working on this gene/RNA

• Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
• Kouji Nakamura, Tsukuba, University, Japan