CheA

• Description: two-component sensor kinase, chemotactic signal modulator
  
  

Gene name	cheA
Synonyms	cheN
Essential	no
Product	two-component sensor kinase
Function	chemotactic signal modulator
MW, pI	74 kDa, 4.452
Gene length, protein length	2013 bp, 671 aa
Immediate neighbours	cheB, cheW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CheA-CheC, CheA-McpB

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P29072

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Travis J Muff, George W Ordal
The CheC phosphatase regulates chemotactic adaptation through CheD.
J Biol Chem: 2007, 282(47);34120-8
[PubMed:17908686] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Michael A Zimmer, Hendrik Szurmant, Michael M Saulmon, Marissa A Collins, Jason S Bant, George W Ordal
The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Mol Microbiol: 2002, 45(2);555-68
[PubMed:12123464] [WorldCat.org] [DOI] (P p)

Liam F Garrity, George W Ordal
Activation of the CheA kinase by asparagine in Bacillus subtilis chemotaxis.
Microbiology (Reading): 1997, 143(Pt 9);2945-2951
[PubMed:12094812] [WorldCat.org] [DOI] (P p)

J R Kirby, C J Kristich, M M Saulmon, M A Zimmer, L F Garrity, I B Zhulin, G W Ordal
CheC is related to the family of flagellar switch proteins and acts independently from CheD to control chemotaxis in Bacillus subtilis.
Mol Microbiol: 2001, 42(3);573-85
[PubMed:11722727] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

M M Rosario, G W Ordal
CheC and CheD interact to regulate methylation of Bacillus subtilis methyl-accepting chemotaxis proteins.
Mol Microbiol: 1996, 21(3);511-8
[PubMed:8866475] [WorldCat.org] [DOI] (P p)

D K Fuhrer, G W Ordal
Bacillus subtilis CheN, a homolog of CheA, the central regulator of chemotaxis in Escherichia coli.
J Bacteriol: 1991, 173(23);7443-8
[PubMed:1938941] [WorldCat.org] [DOI] (P p)