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• Description: DNA-Gyrase (subunit A)
  
  

Gene name	gyrA
Synonyms	nalA
Essential	yes PubMed
Product	DNA-Gyrase (subunit A)
Function	DNA supercoiling, initation of replication cycle and DNA elongation
MW, pI	91 kDa, 5.215
Gene length, protein length	2463 bp, 821 aa
Immediate neighbours	gyrB, rrnO-16S
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00070

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

• Protein family: topoisomerase gyrA/parC subunit family (according to Swiss-Prot)

• Paralogous protein(s): ParC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GyrA-GyrB

• Localization: Nucleoid (Homogeneous) PubMed nucleoid-associated in actively growing cells PubMed

Database entries

• Structure:

• Swiss prot entry: P05653

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: gyrA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, GyrA is subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage