YsxC
- Description: assembly of the 50S subunit of the ribosome
Gene name | ysxC |
Synonyms | orfX |
Essential | yes PubMed |
Product | GTPase |
Function | assembly of the 50S subunit of the ribosome |
Metabolic function and regulation of this protein in SubtiPathways: Stress | |
MW, pI | 21 kDa, 9.708 |
Gene length, protein length | 585 bp, 195 aa |
Immediate neighbours | ysxD, lonA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context |
Contents
The gene
Basic information
- Locus tag: BSU28190
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: engB family (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Swiss prot entry: P38424
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP834 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
Your additional remarks
References
Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945]
[WorldCat.org]
[DOI]
(P p)