PonA

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  • Description: penicillin-binding proteins 1A/1B

Gene name ponA
Synonyms
Essential no
Product penicillin-binding proteins 1A/1B
Function bifunctional glucosyltransferase/ transpeptidase
MW, pI 99 kDa, 4.752
Gene length, protein length 2742 bp, 914 aa
Immediate neighbours recU, ypoC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PonA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU22320

Phenotypes of a mutant

prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed , localizes to the spore septum during sporulation Link to a summary

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
  • Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

Your additional remarks

References

  1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  2. Kawai, Y., R. A. Daniel, J. Errington (2009) Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. Mol. Microbiol. 71: 1131-1144. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed