GntK

• Description: gluconokinase
  
  

Gene name	gntK
Synonyms
Essential	no
Product	gluconokinase
Function	gluconate utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	56 kDa, 5.517
Gene length, protein length	1539 bp, 513 aa
Immediate neighbours	gntR, gntP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-gluconate = ADP + 6-phospho-D-gluconate (according to Swiss-Prot)

• Protein family: FGGY kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P12011

• KEGG entry: [3]

• E.C. number: 2.7.1.12

Expression and regulation

• Operon: gntR-gntK-gntP-gntZ PubMed1PubMed2

• Sigma factor: SigA PubMed

• Regulation:
  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y. (1986) Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis. J Biol Chem. Oct 15;261(29): 13744-53. PubMed
2. Reizer A., Deutscher J., Saier M.H. Jr., Reizer J. (1991) Analysis of the gluconate (gnt) operon of Bacillus subtilis. Mol. Microbiol. 5: 1081-1089. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed