PGP382

From SubtiWiki
Revision as of 18:39, 7 September 2009 by Jstuelk (talk | contribs)
Jump to: navigation, search
pGP382: click to enlarge
pGP382 cloning region: click to enlarge

Vector for the overexpression of C-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP380

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘