cheV

168

modulation of CheA activity in response to attractants

Locus: BSU_14010

Molecular weight: 34.48 kDa

Isoelectric point: 4.62

Protein length: 303 aa

Gene length: 912 bp

Function: control of CheA activity

Product: CheA modulator

Essential: no

Synonyms: cheV

Genomic Context

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List of homologs in different organisms, belongs to COG0835 (Galperin et al., 2021)

Gene

Coordinates: 1,473,605  1,474,516

Phenotypes of a mutant

• ''cheV cheW'' double mutants exhibit complete loss of chemotaxis PubMed
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

The protein

Domains

• N-terminal CheW-like domain, C-terminal two-component receiver domain PubMed
• Response regulatory domain (aa 176-302) (according to UniProt)
• CheW-like domain (aa 16-154) (according to UniProt)

Structure

• P37599 (AlphaFold)

Modification

• the C-terminal two-component receiver domain is phosphorylated on a Asp residue by CheA PubMed

Paralogous protein(s)

• CheW, (N-terminal domain of CheV)

Localization

• forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell PubMed

Expression and Regulation

Operons

• Genes: cheV

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

  Additional information

  • in minimal medium, CheV is present with 7,500 /- 2,000 molecules per cell PubMed

Biological materials

Mutant

• DS70 (cheV::mls in NCIB3610) PubMed
• BKE14010 (cheV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT,  downstream forward: _UP4_TAAATAAAAACAGCCGTTGC
• BKK14010 (cheV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCAATCCCTCGCTATT,  downstream forward: _UP4_TAAATAAAAACAGCCGTTGC

References

Reviews

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Original Publications

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