TrmU

• Description: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
  
  

Gene name	trmU
Synonyms	yrrA, mnmA
Essential	yes PubMed
Product	tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
Function	tRNA modification
Gene expression levels in SubtiExpress: trmU
MW, pI	42 kDa, 4.729
Gene length, protein length	1122 bp, 374 aa
Immediate neighbours	yrrB, yrvO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, membrane proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27500

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU27500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with YrvO) PubMed

• Protein family: mnmA/TRMU family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27500

• Structure:

• UniProt: O35020

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Expression browser: trmU PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 164 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 304 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: