YjbH

• Description: adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents
  
  

Gene name	yjbH
Synonyms
Essential	no
Product	adaptor protein
Function	stimulation of Spx degradation
Gene expression levels in SubtiExpress: yjbH
Interactions involving this protein in SubtInteract: YjbH
MW, pI	31 kDa, 5.206
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	roxS, yjbI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The gene

Basic information

• Locus tag: BSU11550

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of Spx and thus increased expression of trxA PubMed

Database entries

• BsubCyc: BSU11550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: adaptor protein for ClpX-ClpP-catalyzed Spx degradation PubMed

• Protein family: UPF0413 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • contains Zn atoms (coordinated by the N-terminal His-rich region) PubMed

• Effectors of protein activity:
  • YjbH aggregates under stress conditions, resulting in the inability to bind to Spx, and therefore in stabilization of Spx PubMed
  • Zn atom is released upon treatment with strong oxidants PubMed
  • interaction with YirB inhibits the formation of a complex with Spx PubMed

• Interactions:
  • YjbH-Spx PubMed
  • YirB-YjbH PubMed

• Localization: cytosolic protein

Database entries

• BsubCyc: BSU11550

• Structure:

• UniProt: O31606

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbI-yjbH PubMed

• Expression browser: yjbH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between yizD and yjbH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Zuber, Oregon Health and Science University, USA Homepage

Claes von Wachenfeldt, Lund University, Sweden Homepage

References

Original Publications

Jakob Engman, Claes von Wachenfeldt
Regulated protein aggregation: a mechanism to control the activity of the ClpXP adaptor protein YjbH.
Mol Microbiol: 2015, 95(1);51-63
[PubMed:25353645] [WorldCat.org] [DOI] (I p)

Chio Mui Chan, Erik Hahn, Peter Zuber
Adaptor bypass mutations of Bacillus subtilis spx suggest a mechanism for YjbH-enhanced proteolysis of the regulator Spx by ClpXP.
Mol Microbiol: 2014, 93(3);426-38
[PubMed:24942655] [WorldCat.org] [DOI] (I p)

Stephanie Runde, Noël Molière, Anja Heinz, Etienne Maisonneuve, Armgard Janczikowski, Alexander K W Elsholz, Ulf Gerth, Michael Hecker, Kürşad Turgay
The role of thiol oxidative stress response in heat-induced protein aggregate formation during thermotolerance in Bacillus subtilis.
Mol Microbiol: 2014, 91(5);1036-52
[PubMed:24417481] [WorldCat.org] [DOI] (I p)

Nadine Göhring, Iris Fedtke, Guoqing Xia, Ana M Jorge, Mariana G Pinho, Ute Bertsche, Andreas Peschel
New role of the disulfide stress effector YjbH in β-lactam susceptibility of Staphylococcus aureus.
Antimicrob Agents Chemother: 2011, 55(12);5452-8
[PubMed:21947404] [WorldCat.org] [DOI] (I p)

Sushma Kommineni, Saurabh K Garg, Chio Mui Chan, Peter Zuber
YjbH-enhanced proteolysis of Spx by ClpXP in Bacillus subtilis is inhibited by the small protein YirB (YuzO).
J Bacteriol: 2011, 193(9);2133-40
[PubMed:21378193] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Saurabh K Garg, Sushma Kommineni, Luke Henslee, Ying Zhang, Peter Zuber
The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx.
J Bacteriol: 2009, 191(4);1268-77
[PubMed:19074380] [WorldCat.org] [DOI] (I p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
YjbH is a novel negative effector of the disulphide stress regulator, Spx, in Bacillus subtilis.
Mol Microbiol: 2007, 66(3);669-84
[PubMed:17908206] [WorldCat.org] [DOI] (P p)

Annika Rogstam, Jonas T Larsson, Peter Kjelgaard, Claes von Wachenfeldt
Mechanisms of adaptation to nitrosative stress in Bacillus subtilis.
J Bacteriol: 2007, 189(8);3063-71
[PubMed:17293416] [WorldCat.org] [DOI] (P p)