TetB

• Description: multifunctional tetracycline/Na+ resistance protein
  
  

Gene name	tetB
Synonyms	tetA, tetA(L)
Essential	no
Product	multifunctional tetracycline/Na+ resistance protein
Function	resistance to tetracyclin
Gene expression levels in SubtiExpress: tetB
MW, pI	49 kDa, 9.537
Gene length, protein length	1374 bp, 458 aa
Immediate neighbours	yyaP, tetL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

The gene

Basic information

• Locus tag: BSU40770

Database entries

• BsubCyc: BSU40770

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: TCR/tet family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40770

• Structure:

• UniProt: P23054

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tetL-tetB (according to DBTBS)

• Expression browser: tetB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP2080 (tetB::cat), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Wannasiri Wannarat, Shiori Motoyama, Kenta Masuda, Fujio Kawamura, Takashi Inaoka
Tetracycline tolerance mediated by gene amplification in Bacillus subtilis.
Microbiology (Reading): 2014, 160(Pt 11);2474-2480
[PubMed:25169108] [WorldCat.org] [DOI] (I p)

W Wang, A A Guffanti, Y Wei, M Ito, T A Krulwich
Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K(+) acquisition as well as in Na(+), alkali, and tetracycline resistance.
J Bacteriol: 2000, 182(8);2088-95
[PubMed:10735849] [WorldCat.org] [DOI] (P p)

S J Stasinopoulos, G A Farr, D H Bechhofer
Bacillus subtilis tetA(L) gene expression: evidence for regulation by translational reinitiation.
Mol Microbiol: 1998, 30(5);923-32
[PubMed:9988470] [WorldCat.org] [DOI] (P p)

J Cheng, D B Hicks, T A Krulwich
The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange.
Proc Natl Acad Sci U S A: 1996, 93(25);14446-51
[PubMed:8962071] [WorldCat.org] [DOI] (P p)

J Cheng, A A Guffanti, W Wang, T A Krulwich, D H Bechhofer
Chromosomal tetA(L) gene of Bacillus subtilis: regulation of expression and physiology of a tetA(L) deletion strain.
J Bacteriol: 1996, 178(10);2853-60
[PubMed:8631673] [WorldCat.org] [DOI] (P p)

J Cheng, K Baldwin, A A Guffanti, T A Krulwich
Na+/H+ antiport activity conferred by Bacillus subtilis tetA(L), a 5' truncation product of tetA(L), and related plasmid genes upon Escherichia coli.
Antimicrob Agents Chemother: 1996, 40(4);852-7
[PubMed:8849239] [WorldCat.org] [DOI] (P p)

R Sakaguchi, H Amano, K Shishido
Nucleotide sequence homology of the tetracycline-resistance determinant naturally maintained in Bacillus subtilis Marburg 168 chromosome and the tetracycline-resistance gene of B. subtilis plasmid pNS1981.
Biochim Biophys Acta: 1988, 950(3);441-4
[PubMed:2844262] [WorldCat.org] [DOI] (P p)