RuvB

• Description: Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase
  
  

Gene name	ruvB
Synonyms
Essential	no
Product	Holliday junction DNA helicase
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: ruvB
Interactions involving this protein in SubtInteract: RuvB
MW, pI	37 kDa, 5.152
Gene length, protein length	1002 bp, 334 aa
Immediate neighbours	yrzS, ruvA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU27730

Database entries

• BsubCyc: BSU27730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • RuvB-ATP stimulates RecU-mediated Holliday junction resolution PubMed

• Protein family: ruvB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RecU-RuvB PubMed
  • formation of a ternary complex RecU-RuvB-Holliday junction DNA PubMed
  • RuvA-RuvB

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27730

• Structure:

• UniProt: O32055

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ruvA-ruvB PubMed

• Expression browser: ruvB PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A896 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

S C West
Processing of recombination intermediates by the RuvABC proteins.
Annu Rev Genet: 1997, 31;213-44
[PubMed:9442895] [WorldCat.org] [DOI] (P p)

Original publications

Cristina Cañas, Yuki Suzuki, Chiara Marchisone, Begoña Carrasco, Verónica Freire-Benéitez, Kunio Takeyasu, Juan C Alonso, Silvia Ayora
Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution.
J Biol Chem: 2014, 289(25);17634-46
[PubMed:24770420] [WorldCat.org] [DOI] (I p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)