yugT

168

similar to exo-alpha-1,4-glucosidase

Locus: BSU_31290

Molecular weight: 63.82 kDa

Isoelectric point: 4.97

Protein length: 554 aa

Gene length: 1665 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 3.2.1.10

Synonyms: yugT

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

Gene

Coordinates: 3,214,372  3,216,036

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose (according to UniProt)

Protein family

• glycosyl hydrolase 13 family (according to UniProt)

Structure

• 2ZE0 (PDB) (from Geobacillus sp., 64% identity), PubMed
• O05242 (AlphaFold)

Paralogous protein(s)

• TreA, YcdG, MalL

Expression and Regulation

Operons

• Genes: yugT

  Description: PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

Biological materials

Mutant

• MGNA-B547 (yugT::erm), available at the NBRP B. subtilis, Japan
• BKE31290 (yugT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA,  downstream forward: _UP4_TAAACTCCAGCCTTAGACTG
• BKK31290 (yugT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAGCCACTCCTTCCCA,  downstream forward: _UP4_TAAACTCCAGCCTTAGACTG

References

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