LeuA
- Description: 2-isopropylmalate synthase
Gene name | leuA |
Synonyms | |
Essential | no |
Product | 2-isopropylmalate synthase |
Function | biosynthesis of leucine |
Gene expression levels in SubtiExpress: leuA | |
Metabolic function and regulation of this protein in SubtiPathways: leuA | |
MW, pI | 56 kDa, 5.657 |
Gene length, protein length | 1554 bp, 518 aa |
Immediate neighbours | leuB, ilvC |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, membrane proteins, most abundant proteins
This gene is a member of the following regulons
CcpA regulon, CodY regulon, T-box, TnrA regulon
The gene
Basic information
- Locus tag: BSU28280
Phenotypes of a mutant
Database entries
- BsubCyc: BSU28280
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)
- Protein family: LeuA type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot), membrane PubMed
Database entries
- BsubCyc: BSU28280
- Structure: 3EEG (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)
- UniProt: P94565
- KEGG entry: [3]
- E.C. number: 2.3.3.13
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation:
- for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- repressed by casamino acids PubMed
- expression is stimulated in the presence of glucose PubMed
- repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
- repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed
- Regulatory mechanism:
- Additional information:
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
- An antisense RNA is predicted forleuA PubMed
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 1515 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 345 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3390 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1505 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 522 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References