LeuA

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  • Description: 2-isopropylmalate synthase

Gene name leuA
Synonyms
Essential no
Product 2-isopropylmalate synthase
Function biosynthesis of leucine
Gene expression levels in SubtiExpress: leuA
Metabolic function and regulation of this protein in SubtiPathways:
leuA
MW, pI 56 kDa, 5.657
Gene length, protein length 1554 bp, 518 aa
Immediate neighbours leuB, ilvC
Sequences Protein DNA DNA_with_flanks
Genetic context
LeuA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LeuA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins, most abundant proteins

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

  • Locus tag: BSU28280

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA (according to Swiss-Prot)
  • Protein family: LeuA type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3EEG (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY, T-box) PubMed
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed
    • An antisense RNA is predicted forleuA PubMed
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1515 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 345 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3390 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1505 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 522 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References