NfrA

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  • Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein

Gene name nfrA
Synonyms ywcG, ipa-43d
Essential no
Product FMN-containing NADPH-linked nitro/flavin reductase
Function unknown
Gene expression levels in SubtiExpress: nfrA
Metabolic function and regulation of this protein in SubtiPathways:
nfrA
MW, pI 28 kDa, 5.732
Gene length, protein length 747 bp, 249 aa
Immediate neighbours ywcH, rodA
Sequences Protein DNA DNA_with_flanks
Genetic context
NfrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NfrA expression.png




























Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigD regulon, Spo0A regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU38110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed
  • Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 222 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 616 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 560 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 960 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References