IlvH

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  • Description: acetolactate synthase (small subunit)

Gene name ilvH
Synonyms ilvN
Essential no
Product acetolactate synthase (small subunit)
Function biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvH
Metabolic function and regulation of this protein in SubtiPathways:
ilvH
MW, pI 19 kDa, 10.295
Gene length, protein length 522 bp, 174 aa
Immediate neighbours ilvC, ilvB
Sequences Protein DNA DNA_with_flanks
Genetic context
IlvH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IlvH expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

  • Locus tag: BSU28300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: acetolactate synthase small subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2F1F (regulatory subunit from Escherichia coli, 37% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
    • glucose regulation: CcpA PubMed
    • repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
    • TnrA: transcription repression PubMed
    • CodY: transcription repression PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 603 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References