LonA

• Description: class III heat-shock ATP-dependent protease
  
  

Gene name	lonA
Synonyms	lon
Essential	no
Product	class III heat-shock ATP-dependent protease
Function	protein quality control
Gene expression levels in SubtiExpress: lonA
Regulation of this protein in SubtiPathways: lonA
MW, pI	86 kDa, 5.774
Gene length, protein length	2322 bp, 774 aa
Immediate neighbours	ysxC, lonB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, motility and chemotaxis

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

• Locus tag: BSU28200

Database entries

• BsubCyc: BSU28200

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

A mutation in lonA suppresses the motility defect of a lytC mutant PubMed

Evidence for a specific DNA binding activitity of the α-domain was found PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of proteins in presence of ATP (according to Swiss-Prot)

• Protein family: peptidase S16 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • homohexameric protein PubMed
  • LonA-SmiA PubMed
  • LonA-SwrA (degradation) PubMed

• Localization:
  • coincident with the nucleoid during normal growth and localized to the forespore during development PubMed

Database entries

• BsubCyc: BSU28200

• Structure: 3M65 (N-terminal domain) PubMed, 3M6A (C-terminal domain) PubMed, 1X37 (Ssd domain)

• UniProt: P37945

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lonA-ysxC PubMed

• Expression browser: lonA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat stress (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 125 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 660 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Original publications

Sampriti Mukherjee, Anna C Bree, Jing Liu, Joyce E Patrick, Peter Chien, Daniel B Kearns
Adaptor-mediated Lon proteolysis restricts Bacillus subtilis hyperflagellation.
Proc Natl Acad Sci U S A: 2015, 112(1);250-5
[PubMed:25538299] [WorldCat.org] [DOI] (I p)

Ramona E Duman, Jan Löwe
Crystal structures of Bacillus subtilis Lon protease.
J Mol Biol: 2010, 401(4);653-70
[PubMed:20600124] [WorldCat.org] [DOI] (I p)

Yu-Ching Lin, Huai-Cheng Lee, Iren Wang, Chun-Hua Hsu, Jiahn-Haur Liao, Alan Yueh-Luen Lee, Chinpan Chen, Shih-Hsiung Wu
DNA-binding specificity of the Lon protease alpha-domain from Brevibacillus thermoruber WR-249.
Biochem Biophys Res Commun: 2009, 388(1);62-6
[PubMed:19643080] [WorldCat.org] [DOI] (I p)

Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [WorldCat.org] [DOI] (I p)

Lyle A Simmons, Alan D Grossman, Graham C Walker
Clp and Lon proteases occupy distinct subcellular positions in Bacillus subtilis.
J Bacteriol: 2008, 190(20);6758-68
[PubMed:18689473] [WorldCat.org] [DOI] (I p)

E Krüger, M Hecker
The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes.
J Bacteriol: 1998, 180(24);6681-8
[PubMed:9852015] [WorldCat.org] [DOI] (P p)

R Schmidt, A L Decatur, P N Rather, C P Moran, R Losick
Bacillus subtilis lon protease prevents inappropriate transcription of genes under the control of the sporulation transcription factor sigma G.
J Bacteriol: 1994, 176(21);6528-37
[PubMed:7961403] [WorldCat.org] [DOI] (P p)

S Riethdorf, U Völker, U Gerth, A Winkler, S Engelmann, M Hecker
Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
J Bacteriol: 1994, 176(21);6518-27
[PubMed:7961402] [WorldCat.org] [DOI] (P p)