TagD

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  • Description: glycerol-3-phosphate cytidylyltransferase

Gene name tagD
Synonyms
Essential yes PubMed
Product glycerol-3-phosphate cytidylyltransferase
Function biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagD
Metabolic function and regulation of this protein in SubtiPathways:
TagD
MW, pI 15 kDa, 5.672
Gene length, protein length 387 bp, 129 aa
Immediate neighbours tagE, tagA
Sequences Protein DNA DNA_with_flanks
Genetic context
TagD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TagD expression.png
























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

  • Locus tag: BSU35740

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)
  • Protein family: cytidylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 604 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2215 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 751 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 656 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 811 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

A P Bhavsar, T J Beveridge, E D Brown
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.
J Bacteriol: 2001, 183(22);6688-93
[PubMed:11673441] [WorldCat.org] [DOI] (P p)

C H Weber, Y S Park, S Sanker, C Kent, M L Ludwig
A prototypical cytidylyltransferase: CTP:glycerol-3-phosphate cytidylyltransferase from bacillus subtilis.
Structure: 1999, 7(9);1113-24
[PubMed:10508782] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871] [WorldCat.org] [DOI] (P p)