YwjH

• Description: transaldolase
  
  

Gene name	ywjH
Synonyms
Essential	no
Product	transaldolase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ywjH
Metabolic function and regulation of this protein in SubtiPathways: ywjH
MW, pI	22 kDa, 5.876
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	murAB, fbaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU37110

Database entries

• BsubCyc: BSU37110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: Type 3B subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms decamers (dimer of pentamers) PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37110

• Structure: 3R8R PubMed

• UniProt: P19669

• KEGG entry: [3]

• E.C. number: 2.2.1.2

Expression and regulation

• Operon: fbaA-ywjH PubMed

• Expression browser: ywjH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 9591 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 25813 PubMed

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Stülke lab
  • pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Stülke lab
  • GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1407 (spc, based on pGP1331), available in the Stülke lab