pucM

168

uricase

Locus: BSU_32460

Molecular weight: 13.27 kDa

Isoelectric point: 5.08

Protein length: 114 aa

Gene length: 345 bp

Function: purine utilization

Product: uricase

Essential: no

E.C.: 3.5.2.17

Synonyms: pucM, yunM

Genomic Context

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List of homologs in different organisms, belongs to COG2351 (Galperin et al., 2021)

Gene

Coordinates: 3,334,646  3,334,990

The protein

Catalyzed reaction/ biological activity

• 5-hydroxyisourate + H₂O --> 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ (according to UniProt)

Protein family

• transthyretin family (according to UniProt)

Structure

• 2H0E (PDB) PubMed
• O32142 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: pucR-pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • PucR: auto-repression, PubMed, in pucR regulon
  • TnrA: activation, PubMed, in tnrA regulon

Biological materials

Mutant

• MGNA-A938 (yunM::erm), available at the NBRP B. subtilis, Japan
• BKE32460 (pucM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATATGGGTTGTCAGTTTTC,  downstream forward: _UP4_TAAGAAGGAAGCCCGCCTCA
• BKK32460 (pucM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATATGGGTTGTCAGTTTTC,  downstream forward: _UP4_TAAGAAGGAAGCCCGCCTCA

References

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