leuS

168

Leu-tRNA synthetase

Locus: BSU_30320

Molecular weight: 91.35 kDa

Isoelectric point: 4.86

Protein length: 804 aa

Gene length: 2415 bp

Function: translation

Product: leucyl-tRNA synthetase

Essential: yes

E.C.: 6.1.1.4

Synonyms: leuS

Genomic Context

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List of homologs in different organisms, belongs to COG0495 (Galperin et al., 2021)

Gene

Coordinates: 3,102,629 → 3,105,043

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-leucine + tRNA^Leu --> AMP + diphosphate + L-leucyl-tRNA^Leu (according to UniProt)

Protein family

• Class-I aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 1H3N (PDB) (from Thermus thermophilus, 46.4% identity) PubMed
• 2AJG (PDB) (from Escherichia coli, 39% identity, 56% similarity, 22% coverage) PubMed
• P36430 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: leuS

  Description: PubMed

  Regulation

  • induced by leucine limitation (T-box) PubMed

  Regulatory mechanism

  • T-box: RNA switch, via uncharged tRNA-Leu binding to RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

Reviews

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Original Publications

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