sppI

168

antagonist of SppA, stress protein

Locus: BSU_29520

Molecular weight: 18.98 kDa

Isoelectric point: 9

Protein length: 164 aa

Gene length: 495 bp

Function: inhibition of SppA protease activtiy

Product: SppA regulator protein

Essential: no

E.C.: null

Synonyms: sppI, yteJ

Genomic Context

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List of homologs in different organisms, belongs to COG1714 (Galperin et al., 2021)

Gene

Coordinates: 3,019,533  3,020,027

The protein

Catalyzed reaction/ biological activity

• binds to and thereby inhibits SppA PubMed

Domains

• RDD domain (aa 26-158) (according to UniProt)

Structure

• O34424 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: sppA-sppI

  Description: PubMed

  Regulation

  • induced in response to cell wall stress (SigW) PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

  Additional information

  • self-processes its own C-termini PubMed

Biological materials

Mutant

• MGNA-A110 (yteJ::erm), available at the NBRP B. subtilis, Japan
• BKE29520 (sppI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTCGCGTCCATTATTGTTC,  downstream forward: _UP4_TAACCGATAACCCGGACTTC
• BKK29520 (sppI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTCGCGTCCATTATTGTTC,  downstream forward: _UP4_TAACCGATAACCCGGACTTC

References

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