etfA

168

electron transfer flavoprotein (alpha subunit)

Locus: BSU_28520

Molecular weight: 34.39 kDa

Isoelectric point: 4.65

Protein length: 325 aa

Gene length: 978 bp

Function: fatty acid degradation, calcium carbonate biomineralization

Product: electron transfer flavoprotein (alpha subunit)

Essential: no

Synonyms: etfA

Genomic Context

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List of homologs in different organisms, belongs to COG2025 (Galperin et al., 2021)

Gene

Coordinates: 2,915,365  2,916,342

The protein

Protein family

• ETF alpha-subunit/fixB family (single member, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 1EFV (PDB) (the human protein, 39% identity) PubMed
• P94551 (AlphaFold)

Expression and Regulation

Operons

• Genes: lcfA-fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadR-fadB-etfB-etfA

  Description: PubMed

  Regulation

  • induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• 1A856 ( etfA::cat), PubMed, available at BGSC
• BKE28520 (etfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAACATCCCCCTGTT,  downstream forward: _UP4_TAAAGGAAATCCCGGACTTT
• BKK28520 (etfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAACATCCCCCTGTT,  downstream forward: _UP4_TAAAGGAAATCCCGGACTTT

References

Reviews

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Original Publications

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