ysnA

168

ITPase

locus: BSU_28360

Molecular weight: 21.79 kDa

pI: 4.95

Protein length: 198 aa

Gene length: 597 bp

function: purine metabolism

product: ITPase

essential: no

ec: 3.6.1.15

synonyms: ysnA

List of homologs in different organisms, belongs to COG0127 (Galperin et al., 2021)

Gene

Coordinates: 2,900,545  2,901,141

The protein

Catalyzed reaction/ biological activity

• H₂O + XTP --> diphosphate + H⁺ + XMP (according to UniProt)
• dITP + H₂O --> dIMP + diphosphate + H⁺ (according to UniProt)
• H₂O + ITP --> diphosphate + H⁺ + IMP (according to UniProt)

Protein family

• HAM1 NTPase family (single member, according to UniProt)

Structure

• [PDB|2MJP] (from ''Methanococcus jannaschii'') [Pubmed|10404228], [PDB|4BNQ] (from E. Coli 52% identity)
• [AF|P94558]

Expression and Regulation

Operons

• genes: [gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]-[gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]-[gene|13DF13F1EA21BC78035E5ED8B7AF2320235284A6|ysnB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B030 (ysnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1029 NBRP B. subtilis, Japan]
• BKE28360 ([gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT,  downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT
• BKK28360 ([gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT,  downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT

References

• 10404228,11278832