ysnA

168

ITPase

Locus: BSU_28360

Molecular weight: 21.79 kDa

Isoelectric point: 4.95

Protein length: 198 aa

Gene length: 597 bp

Function: purine metabolism

Product: ITPase

Essential: no

E.C.: 3.6.1.15

Synonyms: ysnA

Genomic Context

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Categories containing this gene/protein

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0127 (Galperin et al., 2021)

Gene

Coordinates: 2,900,545  2,901,141

The protein

Catalyzed reaction/ biological activity

H₂O + XTP --> diphosphate + H⁺ + XMP (according to UniProt)
dITP + H₂O --> dIMP + diphosphate + H⁺ (according to UniProt)
H₂O + ITP --> diphosphate + H⁺ + IMP (according to UniProt)

Protein family

HAM1 NTPase family (single member, according to UniProt)

Structure

2MJP (PDB) (from Methanococcus jannaschii) PubMed, 4BNQ (PDB) (from E. Coli 52% identity)
P94558 (AlphaFold)

Expression and Regulation

Operons

Genes: rph-ysnA-ysnB

Description: PubMed

Biological materials

Mutant

MGNA-B030 (ysnA::erm), available at the NBRP B. subtilis, Japan
BKE28360 (ysnA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT,  downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT
BKK28360 (ysnA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT,  downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT

References

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