GcvT

• Description: aminomethyltransferase (glycine cleavage system protein T)
  
  

Gene name	gcvT
Synonyms	yqhI
Essential	no
Product	aminomethyltransferase (glycine cleavage system protein T)
Function	glycine utilization
Gene expression levels in SubtiExpress: gcvT
Metabolic function and regulation of this protein in SubtiPathways: GcvT
MW, pI	39 kDa, 5.734
Gene length, protein length	1086 bp, 362 aa
Immediate neighbours	gcvPA, yqhH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

Gly-box

The gene

Basic information

• Locus tag: BSU24570

Database entries

• BsubCyc: BSU24570

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: [Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH₃ (according to Swiss-Prot)

• Protein family: gcvT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • tetrahydrofolate

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU24570

• Structure: 1YX2

• UniProt: P54378

• KEGG entry: [3]

• E.C. number: 2.1.2.10

Expression and regulation

• Operon: gcvT-gcvPA-gcvPB PubMed

• Expression browser: gcvT PubMed

• Sigma factor:

• Regulation:
  • induced by glycine PubMed

• Regulatory mechanism:
  • induction requires a co-operatively acting riboswitch (Gly-box) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP431 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kokoro Hamachi, Hikari Hayashi, Miyuki Shimamura, Yuiha Yamaji, Ai Kaneko, Aruma Fujisawa, Takuya Umehara, Koji Tamura
Glycols modulate terminator stem stability and ligand-dependency of a glycine riboswitch.
Biosystems: 2013, 113(2);59-65
[PubMed:23721735] [WorldCat.org] [DOI] (I p)

Nathan J Baird, Adrian R Ferré-D'Amaré
Modulation of quaternary structure and enhancement of ligand binding by the K-turn of tandem glycine riboswitches.
RNA: 2013, 19(2);167-76
[PubMed:23249744] [WorldCat.org] [DOI] (I p)

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)