AroF

• Description: chorismate synthase
  
  

Gene name	aroF
Synonyms
Essential	no
Product	chorismate synthase
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroF
Metabolic function and regulation of this protein in SubtiPathways: aroF
MW, pI	40 kDa, 6.578
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	aroB, cheR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

The gene

Basic information

• Locus tag: BSU22710

Database entries

• BsubCyc: BSU22710

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate (according to Swiss-Prot)

• Protein family: acetokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-127 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22710

• Structure: 2O11 (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)

• UniProt: P31104

• KEGG entry: [2]

• E.C. number: 4.2.3.5

Expression and regulation

• Operon:
  • aroF-aroB-aroH (according to DBTBS)
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)

• Expression browser: aroF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4162 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2080 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: