ProJ

• Description: glutamate 5-kinase
  
  

Gene name	proJ
Synonyms	yohA
Essential	no
Product	glutamate 5-kinase
Function	osmoadaptive de novo production of proline
Gene expression levels in SubtiExpress: proJ
Metabolic function and regulation of this protein in SubtiPathways: proJ
MW, pI	40 kDa, 5.296
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	gltC, proH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18470

Database entries

• BsubCyc: BSU18470

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): ProB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU18470

• Structure:

• UniProt: O07509

• KEGG entry: [2]

• E.C. number: 2.7.2.11

Expression and regulation

• Operon: proH-proJ PubMed

• Expression browser: proJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP817 (spc) available in the Stülke lab
  • 1A635 ( proJ::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Adrienne Zaprasis, Monika Bleisteiner, Anne Kerres, Tamara Hoffmann, Erhard Bremer
Uptake of amino acids and their metabolic conversion into the compatible solute proline confers osmoprotection to Bacillus subtilis.
Appl Environ Microbiol: 2015, 81(1);250-9
[PubMed:25344233] [WorldCat.org] [DOI] (I p)

Jeanette Brill, Tamara Hoffmann, Monika Bleisteiner, Erhard Bremer
Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity.
J Bacteriol: 2011, 193(19);5335-46
[PubMed:21784929] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)