AlsT

• Description: amino acid carrier protein
  
  

Gene name	alsT
Synonyms
Essential	no
Product	amino acid carrier protein
Function	amino acid uptake
Gene expression levels in SubtiExpress: alsT
Metabolic function and regulation of this protein in SubtiPathways: alsT
MW, pI	50 kDa, 9.39
Gene length, protein length	1395 bp, 465 aa
Immediate neighbours	ynfC, bglC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, putative amino acid transporter, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU18120

Database entries

• BsubCyc: BSU18120

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sodium:alanine (SAF) symporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane PubMed

Database entries

• BsubCyc: BSU18120

• Structure:

• UniProt: Q45068

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: alsT PubMed

• Sigma factor:

• Regulation:
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • GP1888 (tet) available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)