MtnW

• Description: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein
  
  

Gene name	mtnW
Synonyms	ykrW
Essential	no
Product	2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnW
Metabolic function and regulation of this protein in SubtiPathways: mtnW
MW, pI	44 kDa, 5.284
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	mtnE, mtnX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13590

Database entries

• BsubCyc: BSU13590

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-(methylthio)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (according to Swiss-Prot)

• Protein family: Type IV subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13590

• Structure: 2ZVI PubMed

• UniProt: O31666

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Expression browser: mtnW PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 588 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed