GroEL

• Description: chaperonin and co-repressor for HrcA
  
  

Gene name	groEL
Synonyms
Essential	yes PubMed
Product	chaperonin, co-repressor for HrcA
Function	protein folding and re-folding
Gene expression levels in SubtiExpress: groEL
Interactions involving this protein in SubtInteract: GroEL
Metabolic function and regulation of this protein in SubtiPathways: groEL
MW, pI	57 kDa, 4.531
Gene length, protein length	1632 bp, 544 aa
Immediate neighbours	groES, ydzT/1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU06030

Expression

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU06030

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as co-repressor for HrcA

• Protein family: chaperonin (HSP60) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-35, Arg-116, and Arg-282 PubMed
  • phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions: HrcA-GroEL, GroEL-GroES

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • recruited to the membrane after ethanol stress PubMed

Database entries

• BsubCyc: BSU06030

• Structure:

• UniProt: P28598

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: groES-groEL PubMed

• Expression browser: groEL PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: HrcA: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 14567 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 33441 PubMed

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Jörg Stülke's lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage