GamA

• Description: glucosamine-6-phosphate deaminase
  
  

Gene name	gamA
Synonyms	ybfT
Essential	no
Product	glucosamine-6-phosphate deaminase
Function	glucosamine utilization
Gene expression levels in SubtiExpress: gamA
Metabolic function and regulation of this protein in SubtiPathways: gamA
MW, pI	27 kDa, 5.793
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	gamP, gamR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

GamR regulon

The gene

Basic information

• Locus tag: BSU02360

Phenotypes of a mutant

• no growth on glucosamine PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: d-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃ (according to Swiss-Prot)

• Protein family: NagB subfamily (according to Swiss-Prot)

• Paralogous protein(s): NagB

Extended information on the protein

• Kinetic information: K(M): 3.0 mM PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BKX (the structure of NagB, 50% identity) PubMed

• UniProt: O31458

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: gamA-gamP PubMed

• Expression browser: gamA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by N-acetylglucosamine PubMed
  • induced by glucosamine (GamR) PubMed

• Regulatory mechanism:
  • GamR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabelle Gaugué, Jacques Oberto, Jacqueline Plumbridge
Regulation of amino sugar utilization in Bacillus subtilis by the GntR family regulators, NagR and GamR.
Mol Microbiol: 2014, 92(1);100-15
[PubMed:24673833] [WorldCat.org] [DOI] (I p)

Yanfeng Liu, Long Liu, Hyun-dong Shin, Rachel R Chen, Jianghua Li, Guocheng Du, Jian Chen
Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine.
Metab Eng: 2013, 19;107-15
[PubMed:23876412] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Florence Vincent, Gideon J Davies, James A Brannigan
Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?
J Biol Chem: 2005, 280(20);19649-55
[PubMed:15755726] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

C J BATES, C A PASTERNAK
FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS.
Biochem J: 1965, 96(1);147-54
[PubMed:14343123] [WorldCat.org] [DOI] (P p)