NasB

• Description: nitrate reductase (electron transfer subunit)
  
  

Gene name	nasB
Synonyms	nasBA
Essential	no
Product	nitrate reductase (electron transfer subunit)
Function	utilization of nitrate
Gene expression levels in SubtiExpress: nasB
Interactions involving this protein in SubtInteract: NasB
Metabolic function and regulation of this protein in SubtiPathways: nasB
MW, pI	84 kDa, 5.483
Gene length, protein length	2310 bp, 770 aa
Immediate neighbours	nasC, nasA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU03320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): NasD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • Fe-S cluster PubMed
  • FAD PubMed

• Effectors of protein activity:

• Interactions: NasB-NasC PubMed

• Localization:

Database entries

• Structure:

• UniProt: P42433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nasB-nasC-nasD-nasE-nasF PubMed

• Expression browser: nasB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A971 ( nasB::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

D J Richardson, B C Berks, D A Russell, S Spiro, C J Taylor
Functional, biochemical and genetic diversity of prokaryotic nitrate reductases.
Cell Mol Life Sci: 2001, 58(2);165-78
[PubMed:11289299] [WorldCat.org] [DOI] (P p)

Original publications

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

K Ogawa, E Akagawa, K Yamane, Z W Sun, M LaCelle, P Zuber, M M Nakano
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(5);1409-13
[PubMed:7868621] [WorldCat.org] [DOI] (P p)

M M Nakano, F Yang, P Hardin, P Zuber
Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.
J Bacteriol: 1995, 177(3);573-9
[PubMed:7836289] [WorldCat.org] [DOI] (P p)