HisF

• Description: cyclase-like protein
  
  

Gene name	hisF
Synonyms
Essential	no
Product	cyclase-like protein
Function	biosynthesis of histidine
Gene expression levels in SubtiExpress: hisF
Metabolic function and regulation of this protein in SubtiPathways: hisF
MW, pI	27 kDa, 5.238
Gene length, protein length	756 bp, 252 aa
Immediate neighbours	hisI, hisA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

The gene

Basic information

• Locus tag: BSU34870

Database entries

• BsubCyc: BSU34870

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H₂O (according to Swiss-Prot)

• Protein family: hisA/hisF family (according to Swiss-Prot)

• Paralogous protein(s): HisA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU34870

• Structure:

• UniProt: O34727

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI PubMed

• Expression browser: hisF PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)