GlyA

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  • Description: serine hydroxymethyltransferase

Gene name glyA
Synonyms glyC, ipc-34d
Essential yes PubMed
Product serine hydroxymethyltransferase
Function biosynthesis of glycine
Gene expression levels in SubtiExpress: glyA
Metabolic function and regulation of this protein in SubtiPathways:
glyA
MW, pI 45 kDa, 5.475
Gene length, protein length 1245 bp, 415 aa
Immediate neighbours upp, ywlG
Sequences Protein DNA DNA_with_flanks
Genetic context
GlyA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlyA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

PurR regulon, T-box

The gene

Basic information

  • Locus tag: BSU36900

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine (according to Swiss-Prot)
  • Protein family: SHMT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 2VGU (complex with L-serine, Geobacillus stearothermophilus), 2VI8 (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • induced by glycine limitation (T-box) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References