GyrA

• Description: DNA gyrase (subunit A)
  
  

Gene name	gyrA
Synonyms	nalA
Essential	yes PubMed
Product	DNA gyrase (subunit A)
Function	DNA supercoiling, initation of replication cycle and DNA elongation
Gene expression levels in SubtiExpress: GyrA
Interactions involving this protein in SubtInteract: GyrA
MW, pI	91 kDa, 5.215
Gene length, protein length	2463 bp, 821 aa
Immediate neighbours	gyrB, rrnO-16S
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

The gene

Basic information

• Locus tag: BSU00070

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00070

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to Swiss-Prot)

• Protein family: topoisomerase gyrA/parC subunit family (according to Swiss-Prot)

• Paralogous protein(s): ParC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GyrA-GyrB

• Localization:
  • Nucleoid (Homogeneous) PubMed
  • nucleoid-associated in actively growing cells PubMed

Database entries

• BsubCyc: BSU00070

• Structure:
  • 4DDQ (DNA gyrase A-subunit lacking the C-terminal DNA-wrapping domains) PubMed

• UniProt: P05653

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: gyrA PubMed

• Expression browser: gyrA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, GyrA is subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

References

Martin A Lanz, Mohamad Farhat, Dagmar Klostermeier
The acidic C-terminal tail of the GyrA subunit moderates the DNA supercoiling activity of Bacillus subtilis gyrase.
J Biol Chem: 2014, 289(18);12275-85
[PubMed:24563461] [WorldCat.org] [DOI] (I p)

Markus G Rudolph, Dagmar Klostermeier
Mapping the spectrum of conformational states of the DNA- and C-gates in Bacillus subtilis gyrase.
J Mol Biol: 2013, 425(15);2632-40
[PubMed:23602808] [WorldCat.org] [DOI] (I p)

Luise A K Kleine Borgmann, Hanna Hummel, Maximilian H Ulbrich, Peter L Graumann
SMC condensation centers in Bacillus subtilis are dynamic structures.
J Bacteriol: 2013, 195(10);2136-45
[PubMed:23475963] [WorldCat.org] [DOI] (I p)

Nicole M Baker, Steven Weigand, Sarah Maar-Mathias, Alfonso Mondragón
Solution structures of DNA-bound gyrase.
Nucleic Acids Res: 2011, 39(2);755-66
[PubMed:20870749] [WorldCat.org] [DOI] (I p)

Airat Gubaev, Manuel Hilbert, Dagmar Klostermeier
The DNA-gate of Bacillus subtilis gyrase is predominantly in the closed conformation during the DNA supercoiling reaction.
Proc Natl Acad Sci U S A: 2009, 106(32);13278-83
[PubMed:19666507] [WorldCat.org] [DOI] (I p)

Ulf Gerth, Holger Kock, Ilja Kusters, Stephan Michalik, Robert L Switzer, Michael Hecker
Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis.
J Bacteriol: 2008, 190(1);321-31
[PubMed:17981983] [WorldCat.org] [DOI] (I p)

Thomas Göttler, Dagmar Klostermeier
Dissection of the nucleotide cycle of B. subtilis DNA gyrase and its modulation by DNA.
J Mol Biol: 2007, 367(5);1392-404
[PubMed:17320901] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

W M Huang, J L Libbey, P van der Hoeven, S X Yu
Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation.
Proc Natl Acad Sci U S A: 1998, 95(8);4652-7
[PubMed:9539793] [WorldCat.org] [DOI] (P p)

N Ogasawara, S Moriya, H Yoshikawa
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.
Nucleic Acids Res: 1985, 13(7);2267-79
[PubMed:2987848] [WorldCat.org] [DOI] (P p)