AroE

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  • Description: 3-phosphoshikimate 1-carboxyvinyltransferase

Gene name aroE
Synonyms
Essential no
Product 3-phosphoshikimate 1-carboxyvinyltransferase
Function biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroE
Metabolic function and regulation of this protein in SubtiPathways:
aroE
MW, pI 45 kDa, 6.341
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours ypiA, tyrA
Sequences Protein DNA DNA_with_flanks
Genetic context
AroE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AroE expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

TRAP regulon

The gene

Basic information

  • Locus tag: BSU22600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate (according to Swiss-Prot)
  • Protein family: ABC transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RMT (from B. halodurans, 68% identity, 87% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References