BrxB

• Description: bacilliredoxin
  
  

Gene name	brxB
Synonyms	yqiW
Essential	no
Product	bacilliredoxin
Function	de-bacillithiolation of S-bacillithiolated OhrR and MetE
Gene expression levels in SubtiExpress: brxB
Interactions involving this protein in SubtInteract: BrxB
MW, pI	16 kDa, 4.814
Gene length, protein length	435 bp, 145 aa
Immediate neighbours	artP, bmrU
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU23990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • de-bacillithiolation of S-bacillithiolated OhrR and MetE PubMed

• Protein family: UPF0403 family (according to Swiss-Prot)

• Paralogous protein(s): BrxA

Extended information on the protein

• Kinetic information:

• Domains: contains an CxC redox motif redox active Cys residue [3]

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • BrxB-MetE PubMed
  • BrxB-OhrR PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54534

• KEGG entry: [4]

• E.C. number:

Expression and regulation

• Operon: brxB (according to DBTBS)

• Expression browser: brxB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Your additional remarks

YpdA, BrxA, BrxB and YtxJ cooccur in those species predicted to synthesize bacillithiol.

References

Ahmed Gaballa, Bui Khanh Chi, Alexandra A Roberts, Dörte Becher, Chris J Hamilton, Haike Antelmann, John D Helmann
Redox regulation in Bacillus subtilis: The bacilliredoxins BrxA(YphP) and BrxB(YqiW) function in de-bacillithiolation of S-bacillithiolated OhrR and MetE.
Antioxid Redox Signal: 2014, 21(3);357-67
[PubMed:24313874] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)