GerKB

• Description: nutrient receptor
  
  

Gene name	gerKB
Synonyms
Essential	no
Product	nutrient receptor
Function	germination
Gene expression levels in SubtiExpress: gerKB
Interactions involving this protein in SubtInteract: GerKB
MW, pI	41 kDa, 8.921
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	gerKC, yclH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU03720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:
  • amino acid, polyamine, organocation (APC) superfamily of transporters, TC 2.A.3

• Paralogous protein(s): GerAB, GerBB, YndE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerKA-GerKB-GerKC (tentative receptor complex) PubMed
  • GerKB is part of the germinosome in the spore inner membrane PubMed

• Localization:
  • spore inner membrane PubMed

Database entries

• Structure:

• UniProt: P49940

• KEGG entry: [3]

• T.C. number: 2.A.3.9.3

Expression and regulation

• Operon: gerKA-gerKC-gerKB (according to DBTBS)

• Expression browser: gerKB PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed during sporulation (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Jing-qiao Zhang, Keren K Griffiths, Ann Cowan, Peter Setlow, Ji Yu
Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination.
J Bacteriol: 2013, 195(8);1735-40
[PubMed:23396907] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Takao Igarashi, Peter Setlow
Transcription of the Bacillus subtilis gerK operon, which encodes a spore germinant receptor, and comparison with that of operons encoding other germinant receptors.
J Bacteriol: 2006, 188(11);4131-6
[PubMed:16707705] [WorldCat.org] [DOI] (P p)

Swaroopa Atluri, Katerina Ragkousi, Donna E Cortezzo, Peter Setlow
Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor.
J Bacteriol: 2006, 188(1);28-36
[PubMed:16352818] [WorldCat.org] [DOI] (P p)