CycB

• Description: galactotriose ABC transporter, binding protein
  
  

Gene name	cycB
Synonyms	yvfK
Essential	no
Product	galactotriose ABC transporter, binding protein
Function	uptake of galactotriose
Gene expression levels in SubtiExpress: cycB
Interactions involving this protein in SubtInteract: CycB
MW, pI	45 kDa, 5.441
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	ganP, lacR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34160

Phenotypes of a mutant

• a cycB-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

Database entries

• BsubCyc: BSU34160

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)

• Paralogous protein(s): MdxE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (GanP-GanQ)-CycB PubMed

• Localization: cell membrane (via GanP-GanQ) PubMed

Database entries

• BsubCyc: BSU34160

• Structure:

• UniProt: O07009

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: cycB-ganP-ganQ-ganA-ganB PubMed

• Expression browser: cycB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Pascale B Beauregard, Hera Vlamakis, Richard Losick, Roberto Kolter
Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis.
mBio: 2012, 3(4);e00184-12
[PubMed:22893383] [WorldCat.org] [DOI] (I e)

A Kamionka, M K Dahl
Bacillus subtilis contains a cyclodextrin-binding protein which is part of a putative ABC-transporter.
FEMS Microbiol Lett: 2001, 204(1);55-60
[PubMed:11682178] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)