Des

• Description: phospholipid desaturase
  
  

Gene name	des
Synonyms	yocE
Essential	no
Product	phospholipid desaturase
Function	adaptation of membrane fluidity at low temperatures
Gene expression levels in SubtiExpress: des
Interactions involving this protein in SubtInteract: Des
Metabolic function and regulation of this protein in SubtiPathways: des
MW, pI	40 kDa, 9.88
Gene length, protein length	1056 bp, 352 aa
Immediate neighbours	yocD, desK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

lipid metabolism/ other, cold stress proteins, membrane proteins

This gene is a member of the following regulons

DesR regulon

The gene

Basic information

• Locus tag: BSU19180

Phenotypes of a mutant

• • cold-sensitive PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type, this allows growth at low pressure PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: introduces double bonds into long chain fatty acids PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • Des receives electrons from ferredoxin (Fer) or from either of the two flavodoxins, YkuN or YkuP, the three proteins can functionally replace each other PubMed

• Effectors of protein activity:

• Interactions:
  • Des-Fer (electron transfer to Des) PubMed
  • Des-YkuN (electron transfer to Des) PubMed
  • Des-YkuP (electron transfer to Des) PubMed

• Localization: integral membrane protein PubMed

Database entries

• Structure:

• UniProt: O34653

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: des PubMed

• Expression browser: des PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced upon cold shock (DesR) PubMed
  • repressed at increased membrane fluidity PubMed

• Regulatory mechanism:
  • two component system DesR-DesK: transcription activation PubMed, PubMed

• Additional information:
  • in strains evolved for growth at low pressure, des expression is significantly increased as compared to the wild type PubMed

Biological materials

• Mutant:
  • 1A835 ( des::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage

References

Reviews

John Shanklin, Jodie E Guy, Girish Mishra, Ylva Lindqvist
Desaturases: emerging models for understanding functional diversification of diiron-containing enzymes.
J Biol Chem: 2009, 284(28);18559-63
[PubMed:19363032] [WorldCat.org] [DOI] (P p)