Aag

• Description: general stress protein, similar to DNA-3-methyladenine glycosidase II, required for protection against paraquat stress
  
  

Gene name	aag
Synonyms	yxlJ
Essential	no
Product	unknown
Function	survival of stress conditions
Gene expression levels in SubtiExpress: aag
MW, pI	21 kDa, 6.117
Gene length, protein length	588 bp, 196 aa
Immediate neighbours	yxzF, katX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU38620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed

• Protein family: DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94378

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: aag-yxzF PubMed

• Expression browser: aag PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Alexander Reder, Dirk Höper, Ulf Gerth, Michael Hecker
Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.
J Bacteriol: 2012, 194(14);3601-10
[PubMed:22582280] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Randi M Aamodt, Pål Ø Falnes, Rune F Johansen, Erling Seeberg, Magnar Bjørås
The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates.
J Biol Chem: 2004, 279(14);13601-6
[PubMed:14729667] [WorldCat.org] [DOI] (P p)