YqeL

• Description: ribosomal silencing factor
  
  

Gene name	yqeL
Synonyms
Essential	no
Product	ribosomal silencing factor
Function	translation
Gene expression levels in SubtiExpress: yqeL
Interactions involving this protein in SubtInteract: YqeL
MW, pI	13 kDa, 4.367
Gene length, protein length	354 bp, 118 aa
Immediate neighbours	yqeM, yqeK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU25620

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining PubMed

• Protein family: Iojap family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RplN-YqeL PubMed, the interaction serves to silence translation PubMed

• Localization:

Database entries

• Structure:

• UniProt: P54457

• KEGG entry: [2]

• E.C. number:

Additional information

• the protein is enriched in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed

Expression and regulation

• Operon:

• Expression browser: yqeL PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ningning Li, Yuling Chen, Qiang Guo, Yixiao Zhang, Yi Yuan, Chengying Ma, Haiteng Deng, Jianlin Lei, Ning Gao
Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.
Nucleic Acids Res: 2013, 41(14);7073-83
[PubMed:23700310] [WorldCat.org] [DOI] (I p)

Roman Häuser, Markus Pech, Jaroslaw Kijek, Hiroshi Yamamoto, Björn Titz, Florian Naeve, Andrey Tovchigrechko, Kaori Yamamoto, Witold Szaflarski, Nono Takeuchi, Thorsten Stellberger, Markus E Diefenbacher, Knud H Nierhaus, Peter Uetz
RsfA (YbeB) proteins are conserved ribosomal silencing factors.
PLoS Genet: 2012, 8(7);e1002815
[PubMed:22829778] [WorldCat.org] [DOI] (I p)