SplB

• Description: spore photoproduct lyase
  
  

Gene name	splB
Synonyms	spl
Essential	no
Product	spore photoproduct lyase
Function	protection of spore DNA against photodamage
Gene expression levels in SubtiExpress: splB
MW, pI	39 kDa, 8.524
Gene length, protein length	1026 bp, 342 aa
Immediate neighbours	splA, ykwB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU13930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 4FHC (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed

• UniProt: P37956

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: splA-splB PubMed

• Expression browser: splB PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:
  • negative autoregulation by SplA PubMed

• Additional information:

Biological materials

• Mutant: BP130 (del splB::spc), available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Alhosna Benjdia
DNA photolyases and SP lyase: structure and mechanism of light-dependent and independent DNA lyases.
Curr Opin Struct Biol: 2012, 22(6);711-20
[PubMed:23164663] [WorldCat.org] [DOI] (I p)

Structure of the spore photoproduct lesion in DNA

Isha Singh, Yajun Jian, Lei Li, Millie M Georgiadis
The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition.
Acta Crystallogr D Biol Crystallogr: 2014, 70(Pt 3);752-9
[PubMed:24598744] [WorldCat.org] [DOI] (I p)

Original publications

Linlin Yang, Lei Li
The enzyme-mediated direct reversal of a dithymine photoproduct in germinating endospores.
Int J Mol Sci: 2013, 14(7);13137-53
[PubMed:23799365] [WorldCat.org] [DOI] (P e)

Linlin Yang, Renae S Nelson, Alhosna Benjdia, Gengjie Lin, Joshua Telser, Stefan Stoll, Ilme Schlichting, Lei Li
A radical transfer pathway in spore photoproduct lyase.
Biochemistry: 2013, 52(18);3041-50
[PubMed:23607538] [WorldCat.org] [DOI] (I p)

Alhosna Benjdia, Korbinian Heil, Thomas R M Barends, Thomas Carell, Ilme Schlichting
Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme.
Nucleic Acids Res: 2012, 40(18);9308-18
[PubMed:22761404] [WorldCat.org] [DOI] (I p)

Lei Li
Mechanistic studies of the radical SAM enzyme spore photoproduct lyase (SPL).
Biochim Biophys Acta: 2012, 1824(11);1264-77
[PubMed:22197590] [WorldCat.org] [DOI] (P p)

Linlin Yang, Gengjie Lin, Degang Liu, Karl J Dria, Joshua Telser, Lei Li
Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.
J Am Chem Soc: 2011, 133(27);10434-47
[PubMed:21671623] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

M Pedraza-Reyes, F Gutiérrez-Corona, W L Nicholson
Temporal regulation and forespore-specific expression of the spore photoproduct lyase gene by sigma-G RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1994, 176(13);3983-91
[PubMed:8021181] [WorldCat.org] [DOI] (P p)