IspD

• Description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispD
Synonyms	yacM
Essential	yes PubMed
Product	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispD
MW, pI	25 kDa, 5.579
Gene length, protein length	696 bp, 232 aa
Immediate neighbours	yacL, ispF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigB regulon, SigM regulon

The gene

Basic information

• Locus tag: BSU00900

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)

• Protein family: ispD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2YC3 (from Arabidopsis thaliana, 38% identity) PubMed

• UniProt: Q06755

• KEGG entry: [3]

• E.C. number: 2.7.7.60

Expression and regulation

• Operon: yacL-ispD-ispF PubMed

• Expression browser: ispD PubMed

• Sigma factor: SigB PubMed, SigM PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: