ChaA

• Description: H+/Ca2+ exchanger
  
  

Gene name	chaA
Synonyms	yfkE
Essential	no
Product	calcium export via proton antiporter
Function	calcium export
Gene expression levels in SubtiExpress: chaA
MW, pI	37 kDa, 5.206
Gene length, protein length	1053 bp, 351 aa
Immediate neighbours	yfkF, yfkD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), sporulation proteins, general stress proteins (controlled by SigB), membrane proteins

This gene is a member of the following regulons

SigB regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU07920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms a homotrimer PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure: 4KJS PubMed

• UniProt: O34840

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: chaA-yfkD PubMed

• Expression browser: chaA PubMed

• Sigma factor: SigG, SigB PubMed

• Regulation: expressed in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mousheng Wu, Shuilong Tong, Sandro Waltersperger, Kay Diederichs, Meitian Wang, Lei Zheng
Crystal structure of Ca2+/H+ antiporter protein YfkE reveals the mechanisms of Ca2+ efflux and its pH regulation.
Proc Natl Acad Sci U S A: 2013, 110(28);11367-72
[PubMed:23798403] [WorldCat.org] [DOI] (I p)

Makoto Fujisawa, Yuko Wada, Takahiro Tsuchiya, Masahiro Ito
Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family.
Arch Microbiol: 2009, 191(8);649-57
[PubMed:19543710] [WorldCat.org] [DOI] (I p)