MutTA

• Description: error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
  
  

Gene name	mutTA
Synonyms	ytkD, rppH
Essential	no
Product	8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
Function	DNA repair, RNA degradation
Gene expression levels in SubtiExpress: mutTA
MW, pI	18 kDa, 5.952
Gene length, protein length	474 bp, 158 aa
Immediate neighbours	ytlD, ytkC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins, RNases

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU30630

Database entries

• BsubCyc: BSU30630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
  • RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed

• Protein family: Nudix hydrolase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU30630

• Structure: 4JZV PubMed

• UniProt: O35013

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutTA PubMed

• Expression browser: mutTA PubMed

• Sigma factor: SigA PubMed, SigF PubMed

• Regulation:
  • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Martin Lehnik-Habrink, Richard J Lewis, Ulrike Mäder, Jörg Stülke
RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Mol Microbiol: 2012, 84(6);1005-17
[PubMed:22568516] [WorldCat.org] [DOI] (I p)