PbpI

• Description: sporulation-specific penicillin-binding protein 4b
  
  

Gene name	pbpI
Synonyms	yrrR
Essential	no
Product	penicillin-binding protein 4b
Function	unknown
Gene expression levels in SubtiExpress: pbpI
MW, pI	65 kDa, 9.012
Gene length, protein length	1752 bp, 584 aa
Immediate neighbours	yrrS, greA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigF regulon

The gene

Basic information

• Locus tag: BSU27310

Database entries

• BsubCyc: BSU27310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transpeptidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • may be part of the cell wall biosynthetic complex PubMed

• Localization:

Database entries

• BsubCyc: BSU27310

• Structure:

• UniProt: O32032

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pbpI-yrrS (according to DBTBS)

• Expression browser: pbpI PubMed

• Sigma factor: SigE PubMed, SigF PubMed

• Regulation:
  • expressed early during sporulation in both cell compartments (SigE PubMed, SigF PubMed)

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Dirk-Jan Scheffers
Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 3);999-1012
[PubMed:15758244] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Yuping Wei, Derrell C McPherson, David L Popham
A mother cell-specific class B penicillin-binding protein, PBP4b, in Bacillus subtilis.
J Bacteriol: 2004, 186(1);258-61
[PubMed:14679248] [WorldCat.org] [DOI] (P p)