FatR

• Description: transcriptional repressor of the fatR-yrhJ operon
  
  

Gene name	fatR
Synonyms	yrhI, bscR
Essential	no
Product	transcriptional repressor of the fatR-yrhJ operon (TetR family)
Function	unknown
Gene expression levels in SubtiExpress: fatR
MW, pI	22 kDa, 5.621
Gene length, protein length	582 bp, 194 aa
Immediate neighbours	yrhJ, yrhH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), phosphoproteins

This gene is a member of the following regulons

FatR regulon, SigM regulon, SigW regulon, SigX regulon

The FatR regulon:

• fatR-yrhJ

The gene

Basic information

• Locus tag: BSU27170

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated by PtkA on Tyr-45, this results in displacement of FatR from the DNA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FatR-TkmA PubMed
  • FatR-PtkA PubMed

• Localization:

Database entries

• Structure:

• UniProt: O08335

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • fatR-yrhJ PubMed
  • yrhH-fatR-yrhJ PubMed

• Expression browser: fatR PubMed

• Sigma factor: SigM PubMed, SigW PubMed, SigX PubMed

• Regulation:
  • induced by unsaturated fatty acids (FatR) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • FatR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Abderahmane Derouiche, Vladimir Bidnenko, Rosa Grenha, Nathalie Pigonneau, Magali Ventroux, Mirita Franz-Wachtel, Sylvie Nessler, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Interaction of bacterial fatty-acid-displaced regulators with DNA is interrupted by tyrosine phosphorylation in the helix-turn-helix domain.
Nucleic Acids Res: 2013, 41(20);9371-81
[PubMed:23939619] [WorldCat.org] [DOI] (I p)

Veronica Guariglia-Oropeza, John D Helmann
Bacillus subtilis σ(V) confers lysozyme resistance by activation of two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids.
J Bacteriol: 2011, 193(22);6223-32
[PubMed:21926231] [WorldCat.org] [DOI] (I p)

Min Cao, Tao Wang, Rick Ye, John D Helmann
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons.
Mol Microbiol: 2002, 45(5);1267-76
[PubMed:12207695] [WorldCat.org] [DOI] (P p)

M C Gustafsson, C N Palmer, C R Wolf, C von Wachenfeldt
Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species.
Arch Microbiol: 2001, 176(6);459-64
[PubMed:11734890] [WorldCat.org] [DOI] (P p)

T R Lee, H P Hsu, G C Shaw
Transcriptional regulation of the Bacillus subtilis bscR-CYP102A3 operon by the BscR repressor and differential induction of cytochrome CYP102A3 expression by oleic acid and palmitate.
J Biochem: 2001, 130(4);569-74
[PubMed:11574077] [WorldCat.org] [DOI] (P p)

M C Gustafsson, C von Wachenfeldt
A novel diffusible substance can overcome the apparent AbrB repression of the Bacillus subtilis fatR promoter.
FEMS Microbiol Lett: 2001, 199(2);197-202
[PubMed:11377867] [WorldCat.org] [DOI] (P p)

C N Palmer, M C Gustafsson, H Dobson, C von Wachenfeldt, C R Wolf
Adaptive responses to fatty acids are mediated by the regulated expression of cytochromes P450.
Biochem Soc Trans: 1999, 27(4);374-8
[PubMed:10917605] [WorldCat.org] [DOI] (P p)

X Huang, J D Helmann
Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search.
J Mol Biol: 1998, 279(1);165-73
[PubMed:9636707] [WorldCat.org] [DOI] (P p)