adhB

168

forespore-specific protein, similar to alcohol dehydrogenase

Locus: BSU_26970

Molecular weight: 41.04 kDa

Isoelectric point: 5.66

Protein length: 378 aa

Gene length: 1137 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: adhB

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)

This gene is a member of the following regulons

SigG regulon

Gene

Coordinates: 2,753,452 → 2,754,588

The protein

Protein family

zinc-containing alcohol dehydrogenase family (according to UniProt)

Structure

4CPD (PDB) (from Thermus Sp., 34% identity)
O06012 (AlphaFold)

Modification

CoA is attached to Cys-150 in spores PubMed

Paralogous protein(s)

YycR, (33%),alcohol dehydrogenase

Localization

spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: yraG-yraF-adhB-yraE-yraD

Description: PubMed

Regulation

expressed during sporulation in the forespore (SigG) PubMed

Sigma factors

SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

MGNA-A261 (adhB::erm), available at the NBRP B. subtilis, Japan
BKE26970 (ΔadhB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTAAAACCTCCAGT,  downstream forward: _UP4_CCTTAAAAAACGGAGGTTAC
BKK26970 (ΔadhB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTAAAACCTCCAGT,  downstream forward: _UP4_CCTTAAAAAACGGAGGTTAC

References

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