RipX

• Description: site-specific integrase/recombinase, partitioning of the terminus region after replication
  
  

Gene name	ripX
Synonyms	ykqM
Essential	no
Product	site-specific integrase/recombinase
Function	chromosome partitioning
Gene expression levels in SubtiExpress: ripX
MW, pI	33 kDa, 10
Gene length, protein length	888 bp, 296 aa
Immediate neighbours	drm, yqzK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation

The gene

Basic information

• Locus tag: BSU23510

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)

• Protein family: XerD subfamily (according to Swiss-Prot)

• Paralogous protein(s): CodV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • assembles on the chromosome at the dif site throughout the cell cycle PubMed

Database entries

• Structure:

• UniProt: P46352

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ripX PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A793 ( ripX::spec), PubMed, available at BGSC
  • 1A796 ( ripX::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Alexandra E Bloor, Rocky M Cranenburgh
An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes.
Appl Environ Microbiol: 2006, 72(4);2520-5
[PubMed:16597952] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, G W Blakely
Identification and characterization of the dif Site from Bacillus subtilis.
J Bacteriol: 2001, 183(3);1058-68
[PubMed:11208805] [WorldCat.org] [DOI] (P p)

K P Lemon, I Kurtser, A D Grossman
Effects of replication termination mutants on chromosome partitioning in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2001, 98(1);212-7
[PubMed:11134515] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, D J Sherratt, G Blakely
The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning.
J Bacteriol: 1999, 181(19);6053-62
[PubMed:10498718] [WorldCat.org] [DOI] (P p)